This training is meant specifically for the “Galaxy @Sciensano” that is available at https://galaxy.sciensano.be/ and serves two purposes. First, it serves as an introduction in how to handle and employ the Galaxy instance hosted by Sciensano. Secondly, is also serves as a tutorial into both the basics of next-generation sequencing data analysis, but also more specialized topics of interest in public health (e.g. AMR detection, cgMLST analysis etc., SNP-based outbreak analysis etc.). The training consists specifically out of a series of training videos that are publicly available on YouTube.