Small molecules play an important role in biological systems. They provide the precursors to macromolecules, have roles in cell signalling and regulation, and can be used to treat diseases. To understand the role of small molecules, we can study their binding interactions with macromolecular targets, such as nucleic acids and proteins, and the functional impact on the target and biological pathways. This six-hour virtual workshop will provide participants with an opportunity to gain hands-on experience of using EMBL-EBI tools and resources to assess the impact of drugs on their protein target and place these interactions within the context of biological pathways.During this course, we’ll explore the biological effects of small molecules using a therapeutic drug as an example. We’ll consider a workflow that investigates drug binding to a protein target and the downstream pathway effects. EMBL-EBI tools and resources will be used to identify small molecule drugs, evaluate protein binding and investigate pathway effects.