This course aims to provide participants with theoretical and practical knowledge on how to perform bioinformatic analyses of RNA-seq data using Bioconductor and other open-source software tools. A specific module will briefly describe the single-cell RNA-seq workflow, highlighting differences existing between bulk RNA-seq and single-cell sequencing. The course is suitable for life science researchers who are new to NGS technology, and no statistics prerequisites or computing experience are necessary for attending the course.