The goal of this course is to provide an overview of lab and in-field, real-time nanopore sequencing using the Oxford Nanopore Technologies (ONT) platform.

This course will cover theory of experimental methods and sample collection, plus theory and hands-on experience of DNA extraction and library preparation.

The second part of the course will cover data analysis and visualisation providing hands-on opportunities including the use of QC tools, EI’s MARTi software and tools for Metagenome Assembled Genomes (MAGs), all delivered via virtual machines. You will also have the opportunity to discuss and apply lessons to your own projects with EI’s experts during an optional Q&A session.

What will I learn?

Advantages and disadvantages of different extraction protocols
Familiarity with different DNA QC and library construction methods
Hands-on techniques for preparing samples for nanopore sequencing
Familiarity with nanopore sequencing outputs
Common analysis tools for alignment, assembly and classification of nanopore data
Capabilities of nanopore’s epi2me cloud-based data analysis platform
Effective use of MARTi software for real-time data analysis for species identification and quantification
Tools for assembly of Metagenome Assembled Genomes (MAGs)