Educators:
Dominik Kopczynski (Uni Vienna, LIFS), Nils Hoffmann (FZ Jülich, CAU, LIFS), Dominik Schwudke (FZ Borstel, BioInfra.Prot LIFS)

Location:
On-Site training as part of 9th Lipidomics Forum 2024 in Borstel.

Date & Time:
Sunday, September 1st 2024
10:00 - 16:30

Contents:
In the first part of the course, we will work through an example of targeted LC-MS lipidomics with LipidCreator and Skyline. In the second part of the course, we will provide insights into using LipidXplorer to identify and quantify lipids from shotgun lipidomics. The course will consist of a short theory and background overview of the employed programs complemented by applying the tools to provided data sets. The third part of this course will work through a typical use-case of downstream data processing of shotgun lipidomics data following MS acquisition with LipidXplorer. We will inspect, check, and normalize the data and calculate absolute quantities using internal class-specific standards with lxPostman. We will then perform a qualitative and quantitative comparison of the lipidomes using LipidSpace.

Learning goals:
Participants will be able to understand and explain the targeted shotgun MS and LC-MS workflows for lipidomics. They will learn the fundamentals of the software tools used and how to choose parameters for them. They will learn to understand and interpret the results of each pipeline step.

Prerequisites:
Basic knowledge of lipidomics, analytical workflows in lipidomics, and basic familiarity with web-based and desktop applications. Please note that you may need to install software on your computer to fully participate in all exercises requiring proper rights. The workshop will be a mix of small lecture segments and hands-on exercises. The trainers will be available for questions and assistance during the workshop.

Keywords:
Lipidomics, Shotgun, Targeted Analysis, LC-MSn, Reporting

Tools:
LipidXplorer, LipidCreator, Skyline, Goslin, lxPostman, LipidSpace, Reporting Checklist