Educators:
Jacobo Miranda Ackerman, Fadi Al Machot, Nils Hoffmann (BioInfra.Prot / LIFS)

Date:
January 26th and 27th , 2021

Deadline for Workshop Registration:
January 19th, 2020

Location:
Online, virtual training.

Contents:
In the first part of the course (day 1), we will work through an example for targeted LC-MS lipidomics with LipidCreator and Skyline. In the second part of the course (day 1), we will apply PeakStrainer and LipidXplorer for filtering, identification and quantification of lipid signals from shotgun lipidomics. The course will consist of a short theory and background overview of the employed programs complemented by the application of the tools to provided data sets. The third part of this course (day 2) will work through a typical use-case of downstream data processing of shotgun lipidomics data following MS acquisition with LipidXplorer. We will inspect, check and normalize the data as well as calculate absolute quantities using internal class-specific standards with lxPostman. In part four (day 2), we will then perform a qualitative comparison of the lipidomes using the LUX Score lipidome homology.

Tuesday, January 26th
09:00 – 09:45 Welcome and Introduction
10:00 – 10:30 Targeted Lipidomics with LipidCreator and Skyline
10:30 – 13:00 LipidCreator and Skyline Exercises

Wednesday, January 27th
09:00 – 11:00 Shotgun Lipidomics with LipidXplorer
11:00 – 12:00 Quantification of Shotgun Lipidomics data with lxPostman
12:00 – 13:00 Lipidome comparison with LUX Score

Learning goals:
Participants will be able to understand and explain the shotgun MS and targeted LC-MS workflows for lipidomics. They will learn the fundamentals of the software tools used and how to choose parameters for them. They will learn to understand and interpret the results of each step of the pipeline.

Prerequisites:
Basic knowledge of lipidomics, analytical workflows in lipidomics and basic familiarity with web-based and desktop applications. Please note that you may need to install software on your computer to fully participate in all exercises which may require the proper rights. The workshop will be a mix of small lecture segments and hands-on exercises. The trainers will be available for questions and assistance during the workshop. We will use Zoom to host the workshop. To participate, please ensure that the Zoom client software is installed on your computer.

Keywords:
Lipidomics, Shotgun, Targeted, LC-MS

Tools:
PeakStrainer and LipidXplorer, LipidCreator, Skyline, lxPostman, LUX Score